Analysis of Short Tandem Repeats by Parallel DNA Threading

Analysis of Short Tandem Repeats by Parallel DNA Threading

The majority of studies employing short tandem repeats (STRs) require investigation of several of these genetic markers. As such, we demonstrate the feasibility of the trinucleotide threading (TnT) approach for scalable analysis of STRs. The TnT method represents a parallel amplification alternative that addresses the obstacles associated with multiplex PCR. In this study, analysis of the STR f...

Editorial: Could Speciation Across Evolution be Governed by Genetic Switch Codes at Short Tandem Repeats?

Improved Analysis of DNA Short Tandem Repeats With Time-of-Flight Mass Spectrometry

is currently a research chemist at the National Institute of Standards and Technology and principle investigator on an NIJ-funded project to further develop multiplex PCR and time-of-flight mass spectrometry for future forensic DNA typing assays. He was the first to demonstrate that short tandem repeat typing could be performed with capillary electrophoresis. This document is not intended to cr...

Mutation of human short tandem repeats.

A total of 20,000 parent-offspring transfers of alleles were examined through the genotyping within 40 CEPH reference families of 28 short tandem repeat polymorphisms (STRPs) located on chromosome 19. Forty-seven initial mutation events were detected in the STRPs using DNA from transformed lymphoblastoid cell lines, but less than half (39%) could be verified using DNA from untransformed cells. ...

Forensic Applications of Y-Short Tandem Repeats.

Y-short tandem repeats (STRs) are located in the nonrecombining part of the Y-chromosome. Its genetic characteristics are helpful to paternity testing. The male lineage can be pursued over several generations. Since an overwhelming majority of rape or sexual assault cases involve male suspects, these markers proved to be very useful with mixed stains. Routine casework performed in our laborator...